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Effects of CO2 leakage on the deep living cold water coral Lophelia pertusa - Annual report 2009
To study how leakage from CO2 storage may affect adult survival, metabolism and the reproduction of the deep-sea coral L. pertusa.
White Lophelia pertusa
Project director: Johnsen, Geir, NTNU
Post-doc/ scholar: Järnegren, Johanna
Project duration: 01.01.09-31.12.09
Technical contact person in Statoil: Johnsen, Ståle
Division head: Johnsen, Ståle
Project number: 6153
Phase 1 – Development and testing of chambers, animal tolerance and baseline reproduction. Phase 1 is expected to deliver:
• Two fully operational chambers for study of deep sea organisms under pressure, in particular the effects of CO2
• Experience with the development and use of small pressure chambers for studying deep sea organisms and their ecological tolerance and requirements
• Preliminary results with regard to the effect of pressure on pH, respiration, pigmentation, protein and behaviour on L. pertusa
• Preliminary results with regard to the effect of extreme values of CO2 on respiration, pigmentation and proteins on L. pertusa after short term exposure
• Description of the reproduction and embryo and larval development of L. pertusa
• Determine the possible use of the large chamber (SINTEF/NTNU) for relevant studies of the L. pertusa
Status: According to expected deliveries:
• We have used two chambers, one as a control and one for treatment. There have been difficulties to get the pressure down to the right level as well as having enough flow through the chambers. The units can not be considered as fully operational yet.
• We have further developed the chambers and acquired experience in working with L. pertusa in these.
• We have two experiments conducted, one under 1 atm pressure and one under 40 atm pressure, from which we hope to gain more understanding about the effect of pressure on respiration, pigmentation and proteins after short term exposure. Analyses are currently being conducted on the material.
• In these two experiments pH was lowered in one chamber (pH 7) and we hope to gain more understanding about the effect of pressure on respiration, pigmentation and proteins after short term exposure. Analyses are currently being conducted on the material.
• The reproduction, embryo and larval development have been documented and are currently being described in two articles.
• We have not looked at the possibilities of using the large chamber yet as it has not been operational very long.
• One of two different pigment extraction techniques is finalized, the latter will be finalized in February-March is will be used to give a higher separation of pigments. This work have produced 1 MSc thesis and we are in the finalization state regarding this work.
Publications: Two papers on reproduction, embryo- and larval development of L. pertusa is in preparation. One or two papers will be prepared based on the results from Proteomics and Pigment analysis related to pressure and pH.
Post-doc/ scholar: Järnegren, Johanna
Project duration: 01.01.09-31.12.09
Technical contact person in Statoil: Johnsen, Ståle
Division head: Johnsen, Ståle
Project number: 6153
Phase 1 – Development and testing of chambers, animal tolerance and baseline reproduction. Phase 1 is expected to deliver:
• Two fully operational chambers for study of deep sea organisms under pressure, in particular the effects of CO2
• Experience with the development and use of small pressure chambers for studying deep sea organisms and their ecological tolerance and requirements
• Preliminary results with regard to the effect of pressure on pH, respiration, pigmentation, protein and behaviour on L. pertusa
• Preliminary results with regard to the effect of extreme values of CO2 on respiration, pigmentation and proteins on L. pertusa after short term exposure
• Description of the reproduction and embryo and larval development of L. pertusa
• Determine the possible use of the large chamber (SINTEF/NTNU) for relevant studies of the L. pertusa
Status: According to expected deliveries:
• We have used two chambers, one as a control and one for treatment. There have been difficulties to get the pressure down to the right level as well as having enough flow through the chambers. The units can not be considered as fully operational yet.
• We have further developed the chambers and acquired experience in working with L. pertusa in these.
• We have two experiments conducted, one under 1 atm pressure and one under 40 atm pressure, from which we hope to gain more understanding about the effect of pressure on respiration, pigmentation and proteins after short term exposure. Analyses are currently being conducted on the material.
• In these two experiments pH was lowered in one chamber (pH 7) and we hope to gain more understanding about the effect of pressure on respiration, pigmentation and proteins after short term exposure. Analyses are currently being conducted on the material.
• The reproduction, embryo and larval development have been documented and are currently being described in two articles.
• We have not looked at the possibilities of using the large chamber yet as it has not been operational very long.
• One of two different pigment extraction techniques is finalized, the latter will be finalized in February-March is will be used to give a higher separation of pigments. This work have produced 1 MSc thesis and we are in the finalization state regarding this work.
Publications: Two papers on reproduction, embryo- and larval development of L. pertusa is in preparation. One or two papers will be prepared based on the results from Proteomics and Pigment analysis related to pressure and pH.
Program for the VISTA Day 2009
VISTA Scholars' Day 2010